Shile Huang PhD

The laboratory isprimarily interested in understanding the role of mTOR signaling intumorigenesis and metastasis. mTOR functions as two complexes (mTORC1 andmTORC2), and regulates cell growth, proliferation, survival, and motility. Weare focusing on elucidating how mTOR controls cell motility.

Besides, the laboratory is investigating the molecular mechanisms of anticancer action of small molecules, such as artemisinin and ciclopirox olamine. Artemisinin is a natural product isolated from the plant Artemisia annua, whereas ciclopirox olamine is an off-patent synthetic fungicide. Of note, artesunate (a water-soluble artemisinin derivative) and ciclopirox olamine are undergoing early clinical trials as novel anticancer agents. However, how they execute the anticancer action remains unclear. We are studying the underlying molecular mechanisms.

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ORCID identifier: 0000-0002-3239-1072

MyNCBI Link: https://www.ncbi.nlm.nih.gov/myncbi/shile.huang.1/bibliography/public/?sortby=pubDate&sdirection=descending

Selected Publications:

 
• Luo J, Odaka Y, Huang      Z, Cheng B, Liu W, Li L, Shang C, Zhang C, Wu, Y, Luo Y, Yang S, Houghton      PJ, Guo X, Huang S (2021) Dihydroartemisinin      Inhibits mTORC1 Signaling by Activating the AMPK Pathway in      Rhabdomyosarcoma Tumor Cells. Cells. 10:1363.
 
• Shang C, Zhou H, Liu W, Shen T, Luo Y, Huang S (2020) Iron chelation      inhibits mTORC1 signaling involving activation of AMPK and REDD1/Bnip3      pathways. Oncogene. 39:5201-5213.
 
• Sohretoglu D, Zhang C, Luo J, Huang S (2019) ReishiMax      inhibits mTORC1/2 by activating AMPK and inhibiting IGFR/PI3K/Rheb in      tumor cells. Signal Transduct Target Ther.      4:21.
 
• Barzegar M, Ma S, Zhang C, Chen X, Gu Y, Shang C, Jiang X,      Yang J, Nathan CA, Yang S, Huang      S (2017) SKLB188      inhibits the growth of head      and neck squamous cell carcinoma by suppressing EGFR      signaling. Br J Cancer. 117:1154-1163.
 
• Zhou H, Shang C, Wang M, Shen T, Kong L, Yu      C, Ye Z, Luo Y, Liu L, Li Y, Huang S (2016) Ciclopirox olamine inhibits      mTORC1 signaling by activation of AMPK. Biochem Pharmacol. 116:39-50.
 
• Odaka Y, Xu B, Luo Y, Shen T,      Shang C, Wu Y, Zhou H, Huang S      (2014) Dihydroartemisinin inhibits the mammalian target of      rapamycin-mediated signaling pathways in tumor cells. Carcinogenesis. 35:192-200.
 
• Luo Y, Zhou H, Liu      L, Shen T, Chen W, Xu B, Han X, Alexander JS, Alam A, Huang S (2011) The fungicide ciclopirox inhibits lymphatic      endothelial cell tube formation by suppressing VEGFR-3-mediated ERK      signaling pathway. Oncogene. 30:2098-2107.
 
• Beevers CS, Chen L, Liu L, Luo Y,      Webster NJG, Huang S      (2009) Curcumin disrupts the mammalian target of rapamycin-raptor      complex. Cancer Res.      69:1000-1008.
 
• Liu L, Chen L, Chung J, Huang S (2008) Rapamycin inhibits F-actin reorganization      and phosphorylation of focal adhesion proteins. Oncogene 27:4998-5010.
 
• Liu L, Li F, Cardelli, JA, Martin KA,      Blenis J, Huang S      (2006) Rapamycin inhibits cell motility by suppression of mTOR-mediated      S6K1 and 4E-BP1 pathways. Oncogene. 25:7029-7040.
 
• Huang S, Shu      L, Dilling MB, Easton J, Harwood FC, Ichijo H, Houghton PJ (2003) Sustained activation of the JNK      cascade and rapamycin-induced apoptosis are suppressed by p53/p21^Cip1.      Mol Cell. 11:1491-1501.
 
• Huang S, Liu L, Hosoi H, Dilling MB, Shikata T,      Houghton PJ (2001) p53/p21^CIP1 cooperate in enforcing rapamycin-induced G₁ arrest and determine the cellular      response to rapamycin. Cancer Res.      61:3373-3381.

Keywords/Tags: mTOR, cell motility, cell proliferation, apoptosis, small molecules

Link to lab website:

https://www.lsuhs.edu/departments/school-of-graduate-studies/biochemistry-and-molecular-biology/research/huang-lab

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