Transcriptomics and genomic reorganization during Epstein Barr virus replication and oncogenesis
The Epstein Barr virus (EBV) is a human tumor virus that is a causal agent of some B-cell lymphomas including Burkitt’s lymphoma, diffuse large B-cell lymphoma and Hodgkin’s lymphoma as well as about 10% of stomach cancers. In immunocompromised individuals, EBV associated lymphomas are especially problematic. Our lab investigates the roles of virus latency associated long and short viral non-coding RNAs in contributing to the tumor phenotype through causing global alterations in microRNA function and through eliciting of epigenetic reprogramming. In addition, we study virus-host interactions during the virus replication cycle that lead to extensive remodeling of cell chromatin, the cell epigenome, cell transcription initiation, and splicing disruption in support virus replication and spread within and between hosts.
Education: BS, PhD
ORCID identifier: 0000-0003-0513-5281
MyNCBI link: https://www.ncbi.nlm.nih.gov/myncbi/1XezsqlVF9p5z/bibliography/public/
Selected publications:
- Flemington, EK, Flemington SA, O'Grady TM, Baddoo M, Nguyen T, Dong Y, Ungerleider NA. Splice Tools, a suite of downstream RNA splicing analysis tools to investigate mechanisms and impact of alternative splicing. Nucleic AcidsRes. 2023 Mar 2:gkad111. doi:10.1093/nar/gkad111. Epubahead of print. PMID: 36864749.
- O’Grady TM, Baddoo, M, Flemington, SA, Ishaq, EY, Ungerleider, NA, Flemington, EK. Reversal of splicing infidelity is a pre-activation step in B cell differentiation. Front. Immunol. 2022 Dec 19; DOI 10.3389/fimmu.2022.1060114 PMID:36601126; PMCID: PMC9806119.
- Ungerleider, N, Bullard, W, Kara, M, Wang, X, Roberts, C, Renne, R, Tibbetts, S, and Flemington, EK. EBV microRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escape. PLoS Pathog. 2021 May 6;17(5):e1009217.
doi: 10.1371/journal.ppat.1009217. PMID:33956915; PMCID: PMC8130916.
Topics: Epstein Barr virus, tumor virus, transcriptomics, splicing, transcription, lymphoma, stomach cancer
Link to website: flemingtonlab.tulane.edu
